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Transplex-cwta

TRANSPLEX® C-WTA KIT

The TransPLEX® C-WTA Kit is optimized for the amplification of RNA from difficult samples such as FFPE and serum, providing uniform representation across the entire transcript.

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Product Description

TransPLEX® C-WTA Kit is optimized for the amplification of RNA from difficult samples such as FFPE and serum, providing uniform representation across the entire transcript. The reagent formulations have been designed and optimized to yield a large library from as little as 10 ng of input RNA from fresh tissues and cells or 25 ng from FFPE. The complete TransPLEX C-WTA workflow is performed in a single tube, reducing workflow and minimizing risk of contamination or sample mis-identification.
C-WTA Kit is available for diagnostic use only; cGMP certification ensures ready use for clinical assays

Advantages

  • Proven technology with simple workflow that can be automated
  • Generates isothermal library without 3′-bias followed by amplification
  • Amplifies 10 ng – 300 ng of RNA or formalin-fixed RNA for microarray, PCR, or sequencing assays
  • High success rate (>90%) with FFPE Specimens
  • Manufactured under cGMPs

Specification

Store at –20 °C.
Guaranteed for 12 months at -20°C in a constant temperature freezer.

Kit selection guide

Platform Applications Sample Input Amount Technology
ThruPLEX® DNA-seq Kit Illumina NGS WGS, WES, Targeted Sequencing, ChIP-seq, RNA-seq FFPE DNA, gDNA, cDNA, Cell-free DNA, ChIP DNA, amplicons 50 pg – 50 ng High sensitivity ligation-based amplification
ThruPLEX® Plasma-seq Kit* Illumina NGS WGS, WES, Targeted Sequencing, ctDNA Analysis Cell-free DNA isolated from plasma <1 ng – 30 ng High sensitivity ligation-based amplification
PicoPLEX® DNA-seq Kit Illumina NGS CNV, Aneuploidy Eukaryotic Cell(s) or gDNA <6 pg – 60pg Quasi-random primed linear amplification
PicoPLEX® WGA Kit Arrays, PCR CNV, Aneuploidy Eukaryotic Cell(s) or gDNA <6 pg – 60 pg DNA Quasi-random primed linear amplification
TransPLEX® C-WTA Kit Arrays, PCR Gene Expression Analysis RNA 10 – 300 ng RNA Quasi-random primed amplification