Product Description
The Poly(A) RNA Selection Module enables the rapid and highly specific enrichment of polyadenylated RNAs from total RNA samples.
Poly(A) RNA Selection Kit
Company: Lexogen
- Highly specific poly(A) enrichment
- Magnetic bead-based purification
- Various downstream applications such as RNA-Seq
- Fast workflow
Performance
Rapid Turnaround
Polyadenylated RNAs can be isolated from total RNA samples within about one hour.
Various Downstream Applications
Isolated mRNA can directly be used for RNA-Seq library preparation (e.g., SENSE Total RNA-Seq kit), SAGE, CAGE, cloning, microarrays, cDNA synthesis, and others
Highly Specific for Poly(A) RNA
Other RNA species (rRNA and tRNA) do not contain poly(A) sequences and therefore will not bind to the oligo(dT) beads.
Figure 1. Bioanalyzer traces of the poly(A) RNA enrichment using 5 µg of Universal Human Reference RNA (UHRR) (A) input with the Poly(A) RNA Selection Kit. Recovery of poly(A) selected UHRR (B) is about 2 % of the initial input amo.
Workflow
Step 1 : Preparation of Beads and Denaturation of RNAStep
The total RNA Is briefly denatured and magnetic beads are aliquoted and washed.
Step 2 : Hybridization of poly(A) RNA
The polyadenylated 3’ ends present in most mRNAs are hybridized to oligodT beads.
Step 3 : Poly(A) RNA Enrichment
Any RNA without poly(A) stretches, such as rRNA and tRNA,will not be captured by the oligodT beads and will be washed away.
Step 4 : Poly(A) RNA Elution
The poly(A) RNA is eluted from the olidodT beads by heating in water.
Ordering Information
| Kit | Reactions | Cat.No. |
| Poly(A) RNA Selection Kit | 100 rxn with up to 5 µg total RNA each or 10 rxn with up to 100 µg each | 039.100 |
