Product Description

The Poly(A) RNA Selection Module enables the rapid and highly specific enrichment of polyadenylated RNAs from total RNA samples.

Performance

Rapid Turnaround

Polyadenylated RNAs can be isolated from total RNA samples within about one hour.

Various Downstream Applications

Isolated mRNA can directly be used for RNA-Seq library preparation (e.g., SENSE Total RNA-Seq kit), SAGE, CAGE, cloning, microarrays, cDNA synthesis, and others

Highly Specific for Poly(A) RNA

Other RNA species (rRNA and tRNA) do not contain poly(A) sequences and therefore will not bind to the oligo(dT) beads.

Figure 1. Bioanalyzer traces of the poly(A) RNA enrichment using 5 µg of Universal Human Reference RNA (UHRR) (A) input with the Poly(A) RNA Selection Kit. Recovery of poly(A) selected UHRR (B) is about 2 % of the initial input amo.

Workflow

Step 1 : Preparation of Beads and Denaturation of RNAStep

The total RNA Is briefly denatured and magnetic beads are aliquoted and washed.

Step 2 : Hybridization of poly(A) RNA

The polyadenylated 3’ ends present in most mRNAs are hybridized to oligodT beads.

Step 3 : Poly(A) RNA Enrichment

Any RNA without poly(A) stretches, such as rRNA and tRNA,will not be captured by the oligodT beads and will be washed away.

Step 4 : Poly(A) RNA Elution

The poly(A) RNA is eluted from the olidodT beads by heating in water.

FAQs

Ordering Information

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User Guide – released 26.11.2015
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Material Safety Datasheets

MSDS information for Poly(A) RNA Selection Kit