Product Description
ScreenCell® MB kit is devoted to molecular biology studies. Each kit contains a set of single use DNAse- and RNAse-free filtration devices individually packed, together with a specific buffer; a collection tube is also included.
Before filtration, 6 ml blood samples must be diluted in 1 ml of the ScreenCell® LC dilution buffer, followed 2 minutes later by addition of 1.6 ml of adequate tissue culture medium, allowing isolation of live cells.
At the end of filtration, the nozzle/holder of the ScreenCell® MB is unclipped and removed from the filtration module. The capsule-filter is then ejected and either inserted inside the upper inner part of a nuclease-free Eppendorf® tube, by pushing down a rod located at the bottom part of the filtration device. The capsule-filter may also be ejected into a well of a 24 well tissue culture plate before insertion inside an Eppendorf® tube.
Then the ScreenCell MB filtration unit allows either DNA or RNA extraction directly from cells isolated on the filter before or after cell culture (the cupule-filter containing the cultured cells is then transferred with a nuclease-free forceps into an nuclease-free Eppendorf® tube.
An adequate volume of lysis buffer is added into the capsule-filter which is then closed with the Eppendorf® tube cap in order to extract DNA or RNA directly from cells isolated on the filter. Following incubation at the appropriate temperature, the Eppendorf® tube containing the capsule-filter is centrifuged and the capsule-filter removed and discarded. The flow-through is either stored in the closed Eppendorf® tube or use immediately, to conduct further molecular biology procedures.
Kit content:
– ScreenCell® MB devices
– ScreenCell® LC Buffer
– Collection tube
