Product Description

ThruPLEX® DNA-seq Kit builds on the innovative ThruPLEX chemistry to generate DNA libraries from as little as 50 pg of DNA and increases sensitivity while providing up to 96 indexes for multiplexing. This kit can be used with fragmented DNA from any sample source – genomic, biofluids such as cell-free DNA, DNA from FFPE materials and cDNA. The entire ThruPLEX DNA-seq Kit workflow is performed in a single tube or well in about 2 hours and requires no purification steps or sample transfers. Kits contain 12, 48 or 96 single- or dual-read Illumina-compatible indexes either in tubes (6 or 12) or pre-dispensed and sealed in barcoded microplates. ThruPLEX DNA-seq Kit can be used in any DNA-seq application, RNA-seq,ChIP-seq and offers robust target enrichment performance with all of the leading platforms.

Advantages

• High performance NGS libraries: 50 pg to 50 ng
• Complete package for creating libraries: optimized indexing and adaptor reagents, enzymes, buffers, and nuclease-free water
• Fast and simple workflow: 3 steps in a single tube or well in about 2 hours, no transfers necessary
• Compatible with major target enrichment platforms: Agilent SureSelect ®, Roche Nimblegen ® SeqCap ® EZ, and IDT xGen® Lockdown® probes.

Specification

Store at –20 °C.
Guaranteed for 12 months at -20°C in a constant temperature freezer.

Kit Selection Guide

Performance

ThruPLEX DNA-seq Kit Outperforms Other NGS LIbrary Prep Kits

ThruPLEX DNA-seq Kit yielded more diverse libraries (left) with less input DNA and generated fewer duplicates (right) at 25M read pairs than other kits. NGS libraries were prepared with the indicated amount of reference gDNA using the library preparation kits indicated and sequenced on a MiSeq, v3 flow cell.

ThruPLEX DNA-seq Kit Single-Tube Workflow

Starting with as little as 50 pg of fragmented double-stranded DNA or cDNA, ThruPLEX DNA-seq Kit creates indexed libraries in 3 simple steps: end repair, adapter ligation, and high-fidelity library amplification. The streamlined workflow prevents sample loss and is performed in about 2 hours in a single tube or well preventing sample loss and enhancing positive sample identification.

ThruPLEX DNA-seq Kit Minimizes GC Bias

Relative coverage of the human genome as a function of %GC (50 ng input DNA). (Left) ThruPLEX DNA-seq Kit has excellent coverage throughout the entire range. (Right) ThruPLEX DNA-seq Kit (gold) performs as well as or better than other kits tested. Note that the optimal coverage should be equal to 1 in these graphs. GC composition distribution of the human genome is represented by the red bars.

ThruPLEX DNA-seq Kit Has a Lower and Broader Input Range

ThruPLEX DNA-seq Kit has a lower and broader recommended input range than other kits. The sample input amount for ThruPLEX DNA-seq Kit is 20 times more sensitive than other kits, ranging from 50 pg and 50 ng and requires no optimization of the protocol or adapter concentration. Indexes and adapters are included with the kit.

ThruPLEX DNA-seq Kit Workflow Advantage: High Quality NGS Libraries in About 2 Hours

ThruPLEX DNA-seq Kit converts double-stranded DNA samples to indexed libraries in 3 simple steps: end repair, adapter ligation, and high-fidelity library amplification. The streamlined workflow prevents sample loss and handling errors and is performed in a single tube or well in less than 2 hours.

ThruPLEX DNA-seq Kit Technology

ThruPLEX technology is a 3-step reaction that starts with fragmented double-stranded DNA (0.05 ng to 50 ng) which is repaired in a highly efficient process. Background is reduced using double-stranded adaptors with no single-stranded tails. Blunt end ligation occurs with high-efficiency. Blocked 5’ends reduce adaptor-adaptor ligation. Background is further reduced by destroying unused adaptors after ligation

FAQ

Ordering Information

CAT. NO. R400406 ThruPLEX DNA-seq 48D Kit (48 reactions, 48 dual indexes).
CAT. NO. R400407 ThruPLEX DNA-seq 96D Kit (96 reactions, 96 dual indexes).
CAT. NO. R400427 ThruPLEX DNA-seq 48S Kit (48 reactions, 48 single indexes).
CAT. NO. R400428 ThruPLEX DNA-seq 12S (48) Kit (48 reactions, 12 single indexes).
CAT. NO. R400429 ThruPLEX DNA-seq 12S Kit (12 reactions, 12 single indexes).
CAT. NO. R400523 ThruPLEX DNA-seq 6S (12) Kit (12 reactions, 6 single indexes).

Download

ThruPLEX DNA-seq Kit Instruction Manual

ThruPLEX DNA-seq Product Sheet

ThruPLEX DNA-seq Dual Index Quick Protocol

ThruPLEX DNA-seq Dual Index Plate Guide

ThruPLEX DNA-seq Single Index Quick Protocol

ThruPLEX DNA-seq Single Index Plate Guide

ThruPLEX DNA-seq Index Sequences 6S (CSV)

ThruPLEX DNA-seq Index Sequences 12S (CSV)

ThruPLEX DNA-seq Index Sequences 48S (CSV)

ThruPLEX DNA-seq SDS